早孕期完整胚胎切片的制备方法

Preparation of complete early human embryo sections

  • 摘要: 目的 探讨人类早孕期完整胚胎切片的制备方法,为观察不同时期人类胚胎生殖器官的发育情况及结构变化提供技术平台。 方法 在解剖显微镜下完整取出胎囊内的胎芽与卵黄囊,用湿润滤纸固定,进行常规脱水、渗透、包埋,切片,HE染色后,在显微镜下观察。 结果 早孕胚胎切片完整,清洁,避免了鲜嫩组织的破坏与丢失。 结论 只要操作得当,孕早期胚胎完整切片的获得是完全可能的。

     

    Abstract: Objective To provide the technological platform for observing the development of human embryo reproductive organs and their structure change in different periods by studying the preparation of complete early human embryo sections. Methods Complete embryo and yolk sac were taken out from the gestational sac under anatomical microscope,encased by wetting filter paper,dehydrated,permeated,embedded,sectioned and stained with HE,with their development and structure change observed under microscope. Results The early human embryos were intact and clear with no damage and loss of fresh and tender tissue. Conclusion Complete early human embryo section can be prepared if the manipulation is appropriate.

     

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