大鼠晶状体海水浸泡伤Na+、K+、Ca2+、Mg2+浓度的测定

彭秀军; 何守志; 徐绍娟

大鼠晶状体海水浸泡伤Na⁺、K⁺、Ca²⁺、Mg²⁺浓度的测定

Determination of Na⁺、 K⁺、 Ca²⁺、 Mg²⁺ in the rat lens with seawater immersion

摘要

摘要: 目的: 大鼠晶状体海水浸泡伤后对Na⁺、K⁺、Ca²⁺、Mg²⁺的浓度进行测定并观察和分析其与形态学变化之间的关系。方法: 在晶状体伤后的不同时间采用电感耦合等离子体质谱法测定Na⁺、K⁺、Ca²⁺、Mg²⁺的浓度,同时观察晶状体形态学的变化。结果: 随着海水浸泡时间的延长,Na⁺、Ca²⁺、Mg²⁺浓度逐渐升高,K⁺浓度不断下降。海水浸泡30 m in缝合伤口观察1周组的Na⁺、K⁺、Ca²⁺、Mg²⁺浓度基本恢复正常。海水浸泡30 m in时晶状体明显变混浊,晶状体上皮细胞首先受损,很快发展到皮质层,发生细胞的变性和坏死。结论: 晶状体Na⁺、K⁺、Ca²⁺、Mg²⁺浓度的变化与晶状体组织损伤有着密切的关系。

Abstract: Objective: To determine Na⁺,K⁺,Ca²⁺,Mg²⁺ concentration and simultaneously observe the morphological changes in the rat lens with seawater immersion,and then analysis the relationship between them. Methods: At the different time following seawater immersion,the concentration of Na⁺,K⁺,Ca²⁺,Mg²⁺in rat lenses was determined by ICP-MS.The morphological changes were simultaneously observed. Results: Following the prolongation of the time with seawater immersion,the concentration of Na⁺,Ca²⁺,Mg²⁺ gradually increases,but the concentration of K⁺ gradually decreases in the rat lenses.The concentration of Na⁺,K⁺,Ca²⁺,Mg²⁺ approximately restore normal in the group which the incision were sutured after 30 min with seawater immersion.At 30 min with seawater immersion,lenses were apparently cloudy.The epithelia were early damaged.Further,fiber cells in the cortex occur the degeneration and necrosis. Conclusion: There is close relationship between the changes of Na⁺,K⁺,Ca²⁺,Mg²⁺concentration and the damages of the rat lenses.

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大鼠晶状体海水浸泡伤Na+、K+、Ca2+、Mg2+浓度的测定

Determination of Na+、 K+、 Ca2+、 Mg2+ in the rat lens with seawater immersion

大鼠晶状体海水浸泡伤Na⁺、K⁺、Ca²⁺、Mg²⁺浓度的测定

Determination of Na⁺、 K⁺、 Ca²⁺、 Mg²⁺ in the rat lens with seawater immersion