两种低温保护剂对皮肤储存后α-辅肌动蛋白表达及活力的影响
Effect of two cryoprotectants on expression and viability of α-actinin in human skin
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摘要: 目的 比较两种不同低温保护剂对皮肤组织α-辅肌动蛋白(α-actinin)低温保存后表达的影响,为寻求皮肤组织低温保护剂的最佳配方提供实验依据。方法 新鲜成人皮肤组织分为3组,新鲜对照组和海藻糖-二甲基亚砜(T-D)组、二甲基亚砜-丙二醇(D-P)低温保护剂保存组,-196℃液氮冻存7d、14d复温,免疫组织化学染色对各组间皮肤进行比较。同时对各组皮片进行氧耗量测定。结果 0.5mol/L海藻糖-二甲基亚砜能够很好保护皮肤组织,α-actinin的表达量与新鲜皮肤组相似。结论 海藻糖与二甲基亚砜联合应用对皮肤组织α-actinin的保护作用优于传统组。Abstract: Objective To compare the effects of different cryoprotectants on expression of α-actinin in human skin.MethodsFresh skin tissues were divided into control group,trehalose group,and DMSO group.DMSO and propylene glycol used as cryoprotectants were stored in liquid nitrogen at-196℃ for 7 days and re-warmed after 14 days.The structure of skins in different groups was compared with immunohistochemical staining.Oxygen consumption of skin grafts was measured.ResultsTrehalose and DMSO could protect skin tissues.The expression level of α-actinin in cryopreserved skin tissues was similar to that in fresh skin tissues.ConclusionThe protective effect of trehalose in combination with DMSO on skin tissues is better than traditional drugs.
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