大鼠视神经切开减压和鞘膜切开减压疗效的对比实验

Method of fluorogold retrogradation marker values the effect of optic nerve incision decompression

摘要: 目的: 用荧光金逆行标记法评价视神经切开减压对SD大鼠视神经不完全损伤的疗效。方法: 夹持大鼠左眼视神经30 s建立不完全损伤模型,分视神经切开减压(ONID)和视神经鞘膜切开减压(ONSD)两组,应用荧光金逆行标记法,对比两组在术后1月、2月、3月、6月时存活视神经节细胞(RGC)的数量,评价ONID的疗效。结果: ONID组术后各时间点RGC记数均高于ONSD组,差异有显著性(P<0.05)。结论: ONID对不完全损伤的视神经有保护作用。在延长节细胞存活方面,ONID较传统的ONSD更有效。

Abstract: Objective: To evaluate the curative effect of a new style surgical management,optic nerve incision decompression（ONID）,for optic nerve incomplete injuries by the method of fluorogold retrogradation marker. Methods: By using of reverse tweezer to clamp rat’s left optic nerve,we got mild degree of optic nerve injury model and divided into two groups randomly.After 6 hours of injury,one group was performed by ONID,the other group was performed by optic nerve sheath incision decompression（ONSD）.We counted the number of RGCs in 1month,2months,3months,and 6months. Results: At months1,2,3,6 after decompression the number of RGCs in ONID group were more than that of RGCs in ONSD group significantly（P＜0.05）.（Conclutions:ONID） could protect the incomplete injuried optic nerve.ONID is more efficient than ONSD.