Abstract: Objective: To study the effect of the amount of wear particles on macrophage activation at the bone implant interface.Methods： 1.0mg/ml and 1.5mg/ml （W/V） Ti alloy and CoCr alloy particles suspension was added respectively into synoviocyte system of normal hip joint in vitro. The iof macrophage like cells（MCs） was monitored dynamically by confocal laser scanning microscope （CLSM）. Results： Calcium channels of MCs membrane opened for 1-6 times and caused rapid and transient pulses of iin cytoplasm within 1 hour. There were no significant differences between the times, interval and range of the pulse under the stimulation of different particles and different amount of particles （P＞0.05） But the incubation period of 1.0mg/ml Ti alloy particles group was significantly longer than that of 1.5mg/ml Ti alloy particles group （P＜0.05）. MCs in control group that was stimulated by Hanks’did not appear such reaction Conclusions： Activation of macrophages at boneimplant interface do not correlate with the amount of wear particles while the number of activated macrophages may be relevant to the amount of particles.