嗜酸性粒细胞活化支气管上皮细胞释放IL-6
Epithelial cells activation by co-culture with eosinophils to release IL-6
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摘要: 目的: 探讨嗜酸性粒细胞活化支气管上皮细胞后对其细胞因子白细胞介素(IL)-6分泌的影响。方法: 正常和1%多聚甲醛固定的嗜酸性粒细胞分别与支气管上皮细胞(BEAS-2B)细胞联合培养,应用流式细胞微珠实验(CBA)方法定量细胞培养上清液中细胞因子的浓度,用RT-PCR方法分析经嗜酸性粒细胞活化后支气管上皮细胞中IL-6的基因表达。结果: 经嗜酸性粒细胞活化后,支气管上皮细皮中IL-6基因表达显著上调,细胞培养上清液中IL-6蛋白质浓度升高,多聚甲醛固定后的嗜酸性粒细胞能够刺激支气管上皮细胞中IL-6基因表达和蛋白质释放。结论: 嗜酸性粒细胞可活化支气管上皮细胞中IL-6基因表达和促进其蛋白质分泌。Abstract: Objective:To investigate the effect of human epithelial cells activation by co-culture with human eosinophils on interleukin（IL）-6 expression.Methods:Normal and 1% paraformaldehyde fixed eosinophils were co-cultured with epithelial cells（BEAS-2B cells） respectively;IL-6 gene expression in BEAS-2B cells was evaluated by RT-PCR and IL-6 protein in culture supernatant was determined by flow cytometry with CBA kit.Results:The interaction of eosinophils and BEAS-2B cells was found to up-regulate IL-6 gene expression in BEAS-2B cells and increase IL-6 release in co-culture supernatant.Conclusion:Above results showed that eosinophils can activate epithelial cells to release IL-6.
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