FDP预处理对离体大鼠心脏LVEDP和MDA、SOD的影响

刘福林; 周玉娟; 刘春颖; 武变瑛

FDP预处理对离体大鼠心脏LVEDP和MDA、SOD的影响

Effect of the fructose 1,6-bisphosphate preconditioning in the isolated rat myocardium LVEDP MDA and SOD

摘要

摘要: 目的: 观察1,6-二磷酸果糖(fructose 1,6-b isphosphate,FDP)预处理对离体大鼠心肌组织MDA、SOD的变化,FDP对心肌缺血再灌注损伤的保护效果。方法: 用W istar大鼠建立Langendorff模型,实验随机分为2组,灌注液应用Krebs-R ing液和Krebs-R ing液加FDP 5mmol.L-1两组分别分成缺血前期、缺血期和再灌注期,每8只大鼠1组,共6组。进行对照组和FDP组对比,观察指标左室舒张末压(LVEDP)、心肌组织丙二醛(MDA)的含量、超氧化物歧化酶(SOD)的活性。结果: 对照组大鼠缺血再灌注后LVEDP明显增高(P<0.05)。MDA增高以对照组缺血期最为明显,且对照组缺血期及再灌注期与FDP组缺血期和再灌注期相比较差异显著(P<0.01、P<0.05)。对照组缺血期、再灌期与缺血前期相比较差异明显(P<0.05)。FDP组缺血期、再灌期与缺血前期比较无明显差异。SOD活性FDP组均较对照组增强且具有显著差异(P<0.01)。结论: FDP预处理对心肌具有良好的保护效应,具有明显增加心肌组织对缺氧的...

Abstract: Objective: To study the effect of the fructose 1,6-bisphosphate in preconditioning on the change of myocardium MDA and SOD in the isolated rat. Methods: The experiments were carried out in the ischemia reperfusion model rats.After 10 min preperfusion with Krebs-Ring(with and without 5mmol·L^-1 FDP) and bicarbonate buffer,ischemia time was 20 min,then followed by 20 min reperfusion with K-R buffer(with and without 5mmol·L^-1 FDP).At the end of each step the concentrations of MDA and SOD were determined on ventricle homogenates from 48 hearts of the rats. Results: Control group shows obviously increase LVEDP in the step of ischemia（P＜0.05）.FDP preconditioning could obviously decrease the concentrations of MDA during reperfusion（P＜0.05）,during ischemia（P＜0.01）,increase SOD versus control（P＜0.01）. Conclusion: FDP preconditioning could enhance the effect of root myocardium to hypoxia and have good protective effect on ischemia reperfusion injury of rats myocardium.

HTML全文

参考文献(0)

施引文献

资源附件(0)