Abstract: Objective To study the effect of SV40 gene transfection on ability of lidocaine to transform normal adult liver cells. Methods High-performance liquid chromatography was performed to detect the ability of lidocaine to transform liver cells.Western blotting was used to detect the expression of P450 in liver cells. Results No significant difference was found in the transformation rate of liver cells for lidocaine before and after SV40 gene transfection.Protein 54kU protein bands were observed in liver cells after electrophoresis,indicating that liver cells can express P450 and have immunoreactivity to P4502E. Conclusion Normal human liver cells before and after gene transfection have P450 protein that is necessary for drug metabolism and still maintain the metabolic function of normal adult liver cells.