Abstract: Objective To study the storage of C3A liver cells in a nonfreezing solution at a temperature below zero（-0.8℃） and its relation with cell apoptosis. Methods C3A liver cells suspended in University of Wisconsin（UW） solution were divided into nonfreezing group（-0.8℃） and control group（4℃）.After 24,48 and 72h,viability and apoptosis of liver cells,release of AST and ability of liver cells to synthesize urea and secrete albumin were detected. Results The viability of liver cells was 86.49%±2.80% and 77.83%±3.40% in the nonfreezing and control groups,respectively（P＜0.001）.The apoptosis of liver cells was 1.26%±0.84% and 9.16%±1.99% in the nonfreezing and control groups,respectively（P＜0.001）.The release of AST was significantly suppressed（P＜0.001）.The ability of liver cells to synthesize urea and secrete albumin was better in the nonfreezing group than in the control group（P＜0.001）. Conclusion Storage of C3A hepatocytes in nonfreezing UW solution at a temperature below zero（-0.8℃） can increase the viability of liver cells and their ability to synthesize urea and secrete albumin.