松花粉对脂代谢影响的实验研究
Study of Masson Pine pollen on lipid metabolism in the piglet model
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摘要: 目的: 观察松花粉对家猪脂代谢的影响。方法: 选用雄性阉割猪9头,体重(36±3)kg,随机分为3组:基础饲料组、松花粉添加组和麦麸添加组。分别饲养于单独的代谢笼中,先用普通饲料喂养1周,再给予不同饲料进行代谢实验,测定脂代谢的有关参数。结果: 松花粉组粪便中甘油三酯浓度最高(7.43 mg/g),与其他两组差异显著,每日粪便排出的甘油三酯是麦麸组的3.5倍、基础饲料组的3倍。松花粉组粪便胆固醇浓度也名列第一,每日粪便排出胆固醇总量达735 mg,是基础饲料组(408 mg)的1.7倍、麦麸组(421 mg)的1.5倍。松花粉组每克粪便含脂肪酸总量为15.78 mg(基础饲料组和麦麸组分别为9.07 mg和8.81 mg),是基础饲料组的1.74倍,麦麸组的1.79倍;松花粉组粪便中每日脂肪酸排出总量为1 506 mg,基础饲料组和麦麸组分别为535 mg和560 mg。粪便中胆酸的排泄量松花粉组也高于其他两组,而且与麦麸组间有显著性差异。结论: 食用添加3.5%松花粉饲料后不仅非常显著地增加了家猪粪便中甘油三酯、胆固醇及脂肪酸的排泄量,同时也促进了胆酸的排泄。这一性质对调整机体能量代谢和脂代谢,抑制脂肪的吸收具有重要意义。Abstract: Objective: To observe the effects of Masson Pine pollen on lipid metabolism in the piglet model.Methods: Nine male-castrated growing piglets(bodyweight was 36±3kg) were divided into three groups randomly: basic diet group,wheat bran additive(3.5%) group and pollen additive(3.5%) group.The pigs were housed in metabolic cages individually.After one-week feeding basic diet,then given different diets to observe lipid metabolism for two experimental periods,each period was 14 days.The urine and feces samples were collected,and lipid metabolism parameters were measured.Results: The fecal triglyceride content of pollen group was the highest and had significant statistic difference with other two groups,the daily fecal triglyceride excretion amount was 3.5 times of bran group and 3 times of basic diet group;The cholesterol content of pollen group was the highest too,the daily excretion amount was 735 mg which was 1.54 times of bran group,1.7 times of basic diet group;The fecal total fat acid content of pollen group was 15.78 mg/g,the basic diet group was 9.07 mg/g and bran group was(8.81 mg/g).The daily fecal fat acid excretion amount of pollen group was 1 506 mg,basic diet and bran group were 535 mg and 560mg respectively.Fecal bile acids excretion amount of pollen group was higher than other two groups,and had statistic difference with bran group.Conclusion: Addition of 3.5% Masson Pine pollen significantly increased the fecal triglyceride,cholesterol and total fat acids excretion amount of piglets,and accelerate the bile acids excretion.It has important role in regulation of energy metabolism,lipid metabolism and inhibition of fat absorption.
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