β淀粉样蛋白1-42对小胶质细胞产生一氧化氮作用的实验研究

聂永慧; 王鲁宁; 叶玲; 刘建伟; 刘静

β淀粉样蛋白1-42对小胶质细胞产生一氧化氮作用的实验研究

The study of the production of nitric oxide in β-Amyloid1-42 stimulated microglia in vitro

摘要

摘要: 目的: 应用β淀粉样蛋白1-42(-βAmyloid,Aβ₁-42)作用于小胶质细胞(m icroglia,MG),探讨其产生一氧化氮(n itric oxide,NO)的作用途径。方法: 应用BV-2小胶质细胞作为体外小胶质细胞模型,测定加入Aβ₁-42后细胞上清NO含量及细胞iNOS酶活力;流式细胞仪间接免疫荧光标记法观察Aβ₁-42对iNOS蛋白表达的作用。结果: Aβ₁-42可以刺激BV-2细胞产生NO、提高细胞iNOS酶活性、增加iNOS蛋白质表达,以上作用均具有时间及浓度依赖性。结论: Aβ₁-42在体外可通过活化小胶质细胞,增加NO的表达及分泌,为进一步发挥神经元毒性作用创造条件。

Abstract: Objective: To explore the production of nitric oxide（NO） in β-Amyloid1-42 stimulated microglia in vitro.（Methods: We） cultured murine microglia BV-2 cells as the model of microglia in vitro.The production of nitric oxide（NO） and the activity of inducible nitric oxide synthase（iNOS） were studied in Aβ 1-42 stimulated BV-2 microglia.iNOS protein expression was examined by flow cytometry.Results: NO production was induced by Aβ1-42 in a dose and time dependent manner,Aβ1-42 can also increase iNOS activity and iNOS protein expression in a dose and time dependent manner.iNOS maybe the main source of NO production.Conclusions: On exposure to Aβ microglia can secrete NO,which may trigger pathways of neuronal degeneration in Alzheimer’s disease.amyloid beta-protein;microglia;nitric oxide

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