Abstract: Objective: To investigate the histological outcome of anterior cruciate ligament reconstruction（ACLR） with tendon-knots autograft implant fixation. Methods: Goats were chosen as the animal models.Tendon-knots were press-fitted in the bottleneck-like femoral tunnel as the autografts.Animals were killed at 4,8,12,16,and 24 weeks postoperatively for histological investigation. Results: Histological studies showed incorporation between bone tunnel and tendon-knot graft became dense and mature 12 weeks after the operation. Conclusion: ACLR with tendon-knot press-fitted fixation exhibited satisfied biological results.This research may provide valuable information on clinical application and postoperative rehabilitation.