Abstract: Objective: To explore the possible regulation mechanism of LRP16 gene expression and to clone LRP16 gene promoter molecule, sub promoter molecules and to construct sub-LRP16 gene promoter-pGL3-Basic vectors Methods: A 2.7kb DNA sequence of LRP16 5' end was obtain ed from NCBI by BLAST software. 7 different long target sequences from a healthy blood donor DNA sample were amplified by PCR amplification, then the products w ere identified by DNA sequencing and nest PCR Insert these 7 identified sub LRP16 promoter sequences into-pGL3-Basic vectors. Results: All 7 LRP16 promoter sequences were successfully cloned and 7 sub LRP16 promoter pG L3 Basic vectors were well constructed. Conclusion: A known gene promoter sequence and sub promoter sequence can be freely obtained from NCBI database, and this is very useful for the gene promoter cloning, sub cloning and promoter vector constructing and sub promoter vector constructing