Abstract: Objective To study the changing rule of bone metabolic markers in patients with bone,kidney and cardiovascular diseases and its correlation with routine biochemical parameters. Methods Three bone metabolic markers including osteocalcin（OCN）,β-crosslaps（β-CTx）,parathyroid hormone（PTH）,and 6 routine biochemical parameters including calcium（Ca）,phosphorus（P）,urea（UN）,uric acid（UA）,creatinine（Cr）,alkaline phosphatase（ALP）,and homocysteic acid（HCY） were retrospectively analyzed in 163 patients with bone disease,204 patients with kidney disease,211 patients with cardiovascular disease,and 75 healthy volunteers. Results The β-CTx and PTH level was higher in female patients with bone,kidney and cardiovascular diseases at the age≤50 or ＞50 years than in controls（P＜0.05）.Pearson correlation analysis showed that the β-CTx OCN,and PTH were correlated with ALP,OCN,Cr,and PTH,with a correlation coefficient of 0.361,0.437,0.600,and 0.509,respectively（P＜0.05）. Conclusion Bone metabolic markers can be used not only in diagnosis of bone disease,but also in evaluation of kidney and cardiovascular diseases and cancer.