Background Aeromedical research has found positive acceleration ( + Gx) repeated exposure can cause lung damage, and related mechanisms are still unclear.

Objective To investigate the changes in the expression levels of adhesive plaque-related proteins in rabbit lungs after repeated exposure to + Gx, and explore the underlying mechanisms of + Gx-induced lung injury.

Methods Thirty-two New Zealand white rabbits were randomly divided into control group and experimental group. The experimental group was further divided into three subgroups according to the duration of the experiment (10 days, 20 days, and 30 days), with 8 rabbits in each subgroup. The experimental group was subjected to repeated + Gx acceleration using a horizontal acceleration test platform, with a stable peak acceleration of 4 G, duration of 2 s, and interval of 5 min, for 20 times per day. The control group received the same treatments as the experimental group, without acceleration. After the modeling period, the animals were sacrificed, and lung tissue specimens were collected for immunofluorescence and Western blot analysis to detect the changes in Src, FAK, and Paxillin proteins.

Results Immunofluorescence results showed that compared with the control group, the expression ratio of Src, FAK and Paxillin proteins in the experimental group were increased, and the relative fluorescence intensity of the three proteins in the experimental group increased with the increase in the number of days of the experiment; Western blot results showed that repetitions + Gx acceleration would cause significant increase in rabbit adhesion spot-related proteins FAK, Src and Paxillin (P＜0.05), of which the expression of FAK protein increased with the increase of repetition times, and Src and Paxillin proteins tended to be stable after 20d of exposure.

Conclusion The expression levels of adhesion patch-related proteins in rabbit lung tissues under + Gx acceleration increase significantly, and the expression patterns of different proteins are different with the increase of days of exposure.