CTGF-siRNA转染对血管平滑肌细胞MMP-2、MMP-9表达的影响

罗俊生; 冯旭; 关宁; 霍晓川; 张振兴; 郭闻师

doi:10.3969/j.issn.1005-1139.2012.09.023

CTGF-siRNA转染对血管平滑肌细胞MMP-2、MMP-9表达的影响

Effect of small interference RNA targeting connective tissue growth factor transfection on expression of MMP-2 and MMP-9 in vascular smooth muscle cells

摘要

摘要: 目的通过特异性下调结缔组织生长因子（connective tissue growth factor，CTGF）的表达，观察其对大鼠血管平滑肌细胞中金属蛋白-2（MMP-2）、金属蛋白-9（MMP-9）表达的影响。方法培养大鼠血管平滑肌细胞，免疫组化方法对细胞进行鉴定，用脂质体法将以CTGF第483号编码区为起始设计的小片段干扰核糖核酸（CTGF483-siRNA）对细胞进行转染，Western blot法检测细胞中结缔组织生长因子（CTGF）、MMP-2、MMP-9的表达。结果经免疫组化鉴定，细胞特异性染色呈阳性反应。转染组的CTGF表达明显降低，MMP-2、MMP-9的表达也受到抑制（P<0.05）。结论大鼠血管平滑肌细胞中下调CTGF的表达，可以抑制VSMC中MMP-2、MMP-9的表达，延缓动脉粥样硬化的发展过程，有望成为颈动脉粥样硬化性缺血性脑血管病的预防和治疗新靶点。

Abstract: Objective To observe the effect of connective tissue growth factor(CTGF) on expression of matrix metalloproteinase-2(MMP-2) and MMP-9 in vascular endothelial cells(VSMC). Methods Primary VSMC were cultured and identified with immunohistochemical staining.CTGF483-siRNA was transfected into VSMC with liposome.Expression of CTGF,MMP-2 and MMP-9 in VSMC were detected by Western blot. Results Immunohistochemical staining showed positive reaction in VSMC which were specifically stained.The expression levels of CTGF,MMP-2 and MMP-9 were significantly lower in VSMC after transfection than before transfection(P<0.05). Conclusion Down-regulation of CTGF expression can inhibit the expression of MMP-2 and MMP-9 in VSMC of rats,effectively,delay the development of atherosclerosis,and thus may become a new target for the prevention and treatment of carotid atherosclerotic ischemic cerebral vascular disease.

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