Abstract: Objective To study the relation of fecal Helicobacter pylori（Hp） antigen with gastric cancer and gastrointestinal cancers. Methods Two hundred and four patients were divided into gastric cancer group（n=74）,colon cancer group（n=30）,esophageal cancer group（n=35）,rectal cancer group（n=30）,liver cancer group（n=35） and gastrointestinal cancer group（n=130）.Eighty healthy subjects served as a control group.Fecal Hp antigen in different groups was detected by immune colloidal gold assay.Relation of fecal Hp antigen with gastric cancer and gastrointestinal cancers was analyzed. Results Stool Hp antigen was detected in 74 gastric cancer patients,30 colon cancer patients,35 esophageal cancer patients,30 rectal cancer patients,and 35 liver cancer patients with their age being（57.3±11.6）years,（59.5±12.7）years,（55±9）years,（57.8±12.3）years,and（54.7±11.16）years,respectively.The positive rate of stool Hp antigen was higher in gastric cancer group than in different gastrointestinal cancer groups（43.24% vs 16.61%,2.86%,10%,5.71%） and in healthy group（43.24% vs 1.25%）. Conclusion Stool Hp antigen is closely related with gastric cancer.Immune colloidal gold assay is a simple method for detecting stool Hp antigen.