Abstract: Objective To study the new approaches to liver and pancreas reprogramming by observing the Oct4-mediated dedifferentiation of liver cells into Sox17 positive cells. Methods A lentiviral vector, pWPT-Oct4, was constructed, and lentivirus was packed and concentrated. Primary liver cells in mice were isolated by the modified two-step collagenase perfusion and infected with the concentrated Oct4 lentivirus fluid. Expression of endoderm and hepatic cell transcription factors was detected by RT-PCR. Results Whether the expression vector containing Oct4 lentivirus was successfully constructed was verified by enzyme digestion and sequencing. Exogenous Oct4 was expressed, the expression level of mature transcription factors in liver cells (Alb, Ttr and Afp)decreased, early endoderm marker Sox17 was expressed while endogenous pluripotency genes, Oct4, Nanog, and Sox2 were not expressed in infected primary liver cells. Conclusion Oct4 mediates dedifferentiation of liver cells in which Sox17 positive cells can be observed, thus laying a foundation for the further differentiation of Sox17 positive cells into insulin-producing cells.