Abstract: Objective To provide the evidence for the rational use of drugs by investigating the clinical distribution of 6 983 strains isolated in our hospital from 2008 to 2010 and their drug resistance. Methods The clinical distribution of 6 983 strains isolated in our hospital from 2008 to 2010 and their drug resistance were retrospectively analyzed. Results The mainly isolated Gram-negative bacteria were P. aeruginosa, A.baumannii, E.coli, K. pneumonia and S. maltophilia. The mainly isolated Gram-positive bacteria were staphylococcus aureus, coagulase negative staphylococcus, E. faecalis and E. faecium. The strains were mainly distributed in ICU, departments of respiratory diseases, tuberculosis and neurosurgery, etc. The 3-year ESBL rate of E. coli and K. pneumonia was 47.88% and 38.00%, respectively, and tended to increase yearly. No imipenem-resistant enterobacteriacea strains were found. The drug resistance rates of P. aeruginosa to A. mikacin and pipracillin-tazobatan were relatively low and tended to increase yearly. The drug resistance rates of A.baumannii and S. maltophilia to most antibiotics were relatively high. The total resistance rate of S. aureus strains and coagulase negative staphylococcus to methicillin was 60.29% and 83.43%, respectively. No vancomycin-and linezolidresistant staphylococcal strains were found. The drug resistance rate of E. faecalis strains was lower than that of E. faecium to most tested drugs. A vancomycin-resistant strain was isolated from E. faecium. Conclusion The drug resistance of strains isolated in our hospital from 2008 to 2010 tends to increase and its Monitoring should be strengthened.