异黏蛋白在复发转移性乳腺癌表达及其临床意义的研究

靳鹏; 谢晓冬; 刘兆喆; 宋福林

doi:10.3969/j.issn.2095-5227.2013.02.012

异黏蛋白在复发转移性乳腺癌表达及其临床意义的研究

Expression of metadherin gene in metastatic breast cancer and its clinical significance

摘要

摘要: 目的探讨异黏蛋白（metadherin，MTDH）基因在复发转移乳腺癌患者的表达。方法采用细胞免疫化学方法检测乳腺癌MCF-7、SK-BR3、MDA-MB-231细胞系MTDH基因的表达情况，免疫组化方法检测60例乳腺癌患者术后石蜡组织标本中MTDH基因的表达情况。结果 MTDH在乳腺癌细胞株中主要表达于细胞膜及细胞浆中，MTDH基因在复发转移乳腺癌患者中阳性率为73.8%，而原发乳腺癌患者为38.9%（P< 0.05）。MTDH基因的表达情况与肿瘤的临床分期（P=0.010）、转移部位（P=0.003）、疾病进展时间（time to progression，TTP）（P=0.002）、Ki67（P=0.029）、P53（P=0.022）以及组织分型（P=0.023）有明显的相关性。但与年龄（P=0.970）、原发淋巴结转移（P=0.694）、HER-2（P=0.282）、ER （P=0.056）、PR （P=0.428）等无明显相关性。结论 MTDH基因有望成为乳腺癌患者重要的独立预后因子。

Abstract: Objective To study the expression of metadherin(MTDH) in patients with metastatic breast cancer. Methods Expressions of human breast cancer cell lines MCF-7, SK-BR3 and MDA-MB-231 of MTDH gene were detected with cellular immunochemical method.Expression of MTDH gene was detected in paraffin samples from 60 metastatic breast cancer patients with immunohistochemical method. Results The MTDH gene was mainly expressed in cell membrane or cytoplasm of human breast cancer cell lines.The positive MTDH gene expression rate was significantly higher in metastatic breast cancer patients than in primary breast cancer patients(73.8% vs 38.9%, P< 0.05).The expression of MTDH gene was significantly related with the clinical stage, metastatic site, progress time, Ki67, P53 and histological type of breast cancer(P< 0.05), but not related with the age, primary lymph node metastasis, HER-2, ER, and PR(P> 0.05). Conclusion MTDH gene can be expected to become an independent factor for the prognosis of breast cancer patients

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