Abstract: Objective To study the interference with siRNA JARID1A gene expression on proliferation and apoptosis of Ishikawa cells in endometrial cancer. Methods Ishikawa cells, transfected with JARID1A siRNA, were divided into sicon group, sicon e2 group, siJARIDIA group, and siJARID1A e2 group. Expressions of progestogen receptor (PR) mRNA and protein in the 4 groups were detected by RT-PCR and Western blot, respectively. Proliferation and apoptosis of Ishikawa cells were assayed by MTT and flow cytometry, respectively. Results Interference with JARID1A could significantly up-regulate the PR expression. E2 could further up-regulate the PR expression and promote the proliferation of Ishikawa cells (P< 0.01). The proliferation of Ishikawa cells was inhibited at G1 and G2/M phase 48 h after transfected with siRNA (P< 0.05). Conclusion JARID1A siRNA can effectively inhibit the proliferation and apoptosis of Ishikawa cells.