Abstract: Objective To evaluate the detection methodology of HE4 using electrochemistry luminescent technique (Roche Company). Methods The variation coefficients for within runs and between runs and linear experiment were detected with electrochemistry luminescent technique, and compared with those detected by ELASA. The reliability of electrochemistry luminescent technique was assessed. Results The electrochemistry luminescent technique had a good linearity in the detection range. The variation coefficient for within runs and between runs was 1.05 and 1.83, and 2.63% and 2.68%, respectively, which were well correlated with those detected by ELISA (R²=0.9966). Conclusion The electrochemistry luminescent technique is accurate， reliable, and easy to operate, and is thus worthy to be widely used in clinical practice．