Abstract: Objective To verify the value of single port retroperitoneal laparoscopic radical nephrectomy in locating the renal hilus artery. Methods Sixty patients (40 males, 20 females), at a mean age of 52 years, with a mean BMI of 24.87, who underwent singlesite laparoscopic radical nephrectomy in our hospital from April 2011 to August 2012, were included in this study. CT scanning showed that the longest diameter of tumor was 10.1 cm (mean 5.16 cm). A 5-6 cm incision was made from the posterior axillary line under the lower margin of the 12th rib, into which 2 self-made control loops and a No.7 glove were placed to form the single-port multichannel device. The renal artery was located using the retroperitoneal abdominal wall muscle ligament positioning method for single port laparoscopic radical nephrectomy. Results The single port retroperitoneal laparoscopic radical nephrectomy was successful for all the 60 patients. The kidneys were removed after the renal hilum, renal artery and vein were located under the guidance of medial arcuate ligament and according to the psoas major muscle. The operation time was 1-3 h (mean 131 minutes). No surgical complication occurred in all patients. Conclusion It is safe and feasible to use the self-made single-port multi-channel device in retroperitoneal laparoscopic radical nephrectomy. It is more practical to locate the renal artery by positioning the renal hilus through the abdominal wall muscle ligament in the relatively small operating space of single port retroperitoneal laparoscopic radical nephrectomy.