Abstract: Objective To provide the evidence for clinical treatment of bacterial infection by investigating the isolated bacteria from patients admitted to our hospital in 2012 and their drug resistance. Methods The bacteria were identified and their drug sensitivity was tested with the VITEK microbial analyzer.Their drug resistance was analyzed using the WHONET5.4. Results Among the 2 698 bacterial strains isolated in 2012 from patients in our hospital,the most common microorganism was E.coli(13.8%),followed by P.Aeruginosa(12.4%),C.albicans(12.3%),K.pneumoniae(9.7%),A.baumannii(8.5%),S.aurcus(7.2%),C.glabrata(4.2%),S.epidermidis(3.8%),S.marcescens(3.0%) and E.cloacae(2.4%).Of these bacteria,Gram-negative bacteria,Gram-positive bacteria,fungus,and methicillin resistant staphylococcus aureus(MRSA) accounted for 57.6%,22.9%,19.5% and 65.6%,respectively.The resistance of MRSA to β-lactams and other antimicrobial agents was significantly higher than that of methicillin-sensitive staphylococcus aureus(MSSA).No staphylococcus was sensitive to Vancomycin and linezolid.The resistance of enterococci to vancomycin was 3.8%.The resistance of E.faecalis to most antibiotics was significantly lower than that of E.faecium.The resistance of E.coli to imipenem and meropenem was significantly lower than that of K.pneumoniae(0.6% and 0.8% vs 8.3% and 8.9%).The resistance of P.aeruginosa to imipenem and meropenem was significantly higher than that of cefepime(41.3% and 32.7% vs 20.4%).The resistance of A.baumannii was significantly higher to imipenem,meropenem and different antibiotics than to amikacin(61.8%,61.4%,>50% vs 30.3%).The detection rate of pan-drug resistant P.aeruginosa was 2.1% in 2012.The sensitivity of Candida albicans was the highest to VOR and no resistant Candida albicans strain was isolated. Conclusion Regular bacterial drug resistance monitoring contribute to understanding its change and can thus provide the evidence for rational use of drugs in clinical practice.