淋巴细胞溶质蛋白1调控弥漫性大B细胞淋巴瘤细胞的增殖初探

张竹红; 田亚平

doi:10.3969/j.issn.2095-5227.2013.10.017

淋巴细胞溶质蛋白1调控弥漫性大B细胞淋巴瘤细胞的增殖初探

Lymphocyte cytosolic protein 1 regulates proliferation of diffuse large B-cell lymphoma cells

摘要

摘要: 目的探索弥漫性大B细胞淋巴瘤中淋巴细胞溶质蛋白1 （lymphocyte cytosolic protein 1， LCP1）表达的生物学意义及对细胞增殖的影响。方法用Wstern Blot的方法观察LCP1在正常人B淋巴母细胞系和多种弥漫性大B细胞淋巴瘤细胞系中的表达情况；进而在高表达LCP1的弥漫性大B细胞淋巴瘤（diffuse large B-cell lymphom， DLBCL）细胞系OCI-LY19中将其沉默，使用CCK8试剂盒检测其对细胞活力的影响；并用PI染色的方法检测LCP1对OCI-LY19细胞的细胞周期的影响。结果 LCP1在DLBCL细胞中的表达量明显高于正常人B淋巴母细胞系，沉默LCP1后发现OCI-LY19细胞活力下降，且细胞周期进展明显被抑制。结论 LCP1与DLBCL细胞增殖密切相关，提示其是一个重要DLBCL生物标记。

Abstract: Objective To study the biological significance of lymphocyte cytosolic protein 1(LCP1) expression in diffuse large B-cell lymphoma(DLBCL) cells and its effect on proliferation of DLBCL cells. Methods Expression of LCP1 in healthy human B lymphoblastoid cells and various DLBCL cells was detected by Western blot.Over-expressed LCP1 was silenced in DLBCL cell line OCI-LY19.Vitality of the cells was tested with a CCK8 kit.Effect of LCP1 on cell cycle of OCI-LY19 cells was observed with PI staining. Results The expression level of LCP1 was significantly higher in DLBCL cells than in healthy human B lymphoblastoid cells.The vitality of OCI-LY19 cells decreased and the progressive cell sycle of OCI-LY19 cells was inhibited after LCP1 was silenced. Conclusion LCP1 is closely related with the proliferation of DLBCL cells and is an important biological marker for DLBCL.

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