Abstract: Objective To study the haplo-hematopoietic stem cell transplantation in treatment of aplastic anemia-paroxysmal nocturnal hemoglobinuria syndrome (AA-PNH). Methods One patient with AA-PNH for 3 years admitted to our hospital in March 2011 underwent transplantation of haplo-hematopoietic stem cells from his mother. Grafts were selected from the mobilized haplohematopoietic bone marrow and peripheral blood. Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were transferred as the third part of cells. Results The total number of mononuclear cells (MNC) and CD34⁺cells was 10.8×10⁸/kg and 4.8× 10⁶/kg, respectively. The number of neutrophils and platelets was＞ 0.5×10⁹/L and＞ 20×10⁹/L on days 37 and 58. The chimeras accounted for 100% on day 30 and grade 1 aGVHD was observed on about day 40, with no other severe complications occurred after treatment. Conclusion Haplo-hematopoietic stem cell transplantation is a safe and effective treatment modality for AA-PNH syndrome.