Abstract: Objective To study the effect of HIF1α^mu-tansfected exogenous endothelial progenitor cells (EPCs) on expression of CXCL12. Methods EPCs were isolated, cultured and identifed by density gradient centrifugation in combination with differential velocity adherent technique. Adv-HIF- 1α^muwas transfected into EPCs with the optimum multiplicity of infection (MOI). EPCs containing the purpose gene served as group A, Adv-transfected EPCs served as group B, and non Adv-HIF-1α^mu-transfected EPCs served group C. Expression of HIF-1 in transfected EPCs was detected by Western blot. Effect of CXCL12 concentration in transfected EPCs supernatant on migration of exogenous EPCs was assayed. Results The EPCs isolated by density gradient centrifugation in combination with differential velocity adherent technique can bind to UEA-1 and uptake ac-LDL, and are thus angiogenic. The expression level of HIF-1 and CXCL12 in Adv-HIF-1α^mu-transfected EPCs was signifcantly higher in group A than in groups B and C(P＜ 0.05) and can induce the migration of exogenous EPCs. Conclusion Density gradient centrifugation in combination with differential velocity adherent technique can isolate and culture the characteristic EPCs. Adv-HIF-1α^mu -transfected EPCs can highly express HIF-1 and CXCL12 and induce the migration of exogenous EPCs.