Abstract: Objective To observe the expression changes of genes related to cell cycle after exposing to fluid shear stress (FSS) in human osteosarcoma MG-63 cells. Methods MG-63 cells were cultured for 72 h, and then they were randomly divided into the fluid shear stress group and the control group. The fluid shear stress group was exposed to 1.5 Pa FSS for 60 min, while the control group was treated with no FSS. Then RNA was extracted from the two groups, and the chip was scanned with Affymetrix gene chip and analyzed for differentially expressed genes. Results The expression of genes related to cell cycle in MG-63 cells was changed. Compared with the control group, 247 genes related to cell cycle showed different expression in the FSS group. Of the 247 genes, 130 genes showed up-regulated (Fold Change＞ 2, P＜ 0.05), while the rest of them showed down-regulated (Fold Change＜ -2, P＜ 0.05). These differential genes participated in many cell biological progresses, such as cell cycle process, cell cycle arrest, negative regulation of mitotic cell cycle, G₁/S transition of mitotic cell cycle, interphase of mitotic cell cycle. Conclusion FSS can stimulate the proliferation and differentiation of MG-63 cells via regulating the expression of genes related to cell cycle.