Abstract: Objective To explore the antioxidant capacity of propofol combined vitamin C by observing the change of level of MDA, SOD, T-AOC in serum and brain tissue of KM mice. Methods Fifty male KM mice with age of 6-8 weeks and weight of 20-24 kg were randomly divided into fve groups: 0.9% sodium chloride injection control group, 80 mg/kg propofol (PPF) group, 67 mg/kg P60+Vc group, 100 mg/kg P55+Vc group and 200 mg/kg P50+Vc group, 10 in each group. Rats were injected intraperitoneally with different dose of mixture of propofol and vitamin C, and after 1 min of loss of righting refex (LORR), blood sample from orbital venous and brain tissues were collected, and MDA, SOD, T-AOC in serum and brain of all groups were measured by ELISA. Results Compared with PPF80 group, the MDA level of serum in P60+Vc67, P55+Vc100, P50+Vc200 group was lower (P＜ 0.05), while the SOD, T-AOC level of serum in P60+Vc67, P55+Vc100, P50+Vc200 group was higher (P＜ 0.05). And the MDA level of serum in P55+Vc100, P50+Vc200 group was lower (P＜ 0.05), the SOD, T-AOC level of serum of P60+Vc67, P55+Vc100, P50+Vc200 group was higher (P＜ 0.05). Conclusion Vitamin C can enhance the total antioxidant capacity after KM mice undertaking propofol, which indicates that vitamin C can increase the antioxidant capacity of KM mice after propofol anesthesia.