Abstract: Objective To investigate the changes of autophagy in lung tissues of rats with mutiple trauma． Methods Fifty-six Sprague-Dawley (SD) rats were randomly divided into sham group (n=28) and model group (n=28)． The lower lobe of right lung were taken and the expressions of Beclin-1 and LC3-Ⅱ mRNA in lung tissues of rats were measured by real-time fluorescent polymerase chain reaction (PCR) at 6 h, 12 h, 24 h, 48 h and 5 d after trauma． Results Compared with the sham group, the expressions of LC3-Ⅱand Beclin-1 mRNA in model group increased significantly at different time points． Beclin-1 and LC3-Ⅱ of model group increased significantly than sham group at 48 hours after operation (7．532±0．03) vs (1．063±0．10), (10．843±0．03) vs (1．091±0．11), P< 0． 01． Conclusion Autophagy may be involved in multiple trauma of acute lung injury, like femoral shaft fracture combined with brain injury．