Abstract: Recently, the interaction between osteoclast precursors and osteoclasts in osteroprosis and bone fracture healing has drawn more and more attention, which is considered to be the key therapeutic target in treating osteroprosis and other bone destruction diseases． This paper reviews the mechanism of the bone resorption, particularly focusing on the latest research progress regarding the regulatory function of CX3CL1-CX3CR1 pathways and S1P signal． And it also summarizes the method which involves two-photon microscope observation of osteoclasts and osteoclast precursor in vivo, which is considered to be able to open a new era in the field of orthopedic research．