Abstract: Objective To identify expressions of miR-9 and TMEM16A in glioma T98G cells, explore the role of miR-9 on cell proliferation and invasion of glioma T98G cells and TMEM16A expression. Methods miR-9 which might regulate TMEM16A expression was predicted by bioinformatics and identified with luciferase assay. After transfection of miR-9 mimics into cells, the expressions of miR-9 and TMEM16A were determined by real-time PCR and western blot. The proliferation and invasion of T98G cells were respectively detected in vitro using the colonly formation assay experiment and transwell chamber. Results miRanda and TargetScan showed that miR-9 was well complementary with TMEM16A gene. miR-9 could inhibit TMEM16A mRNA expression shown by luciferase assay. Results of real-time PCR and western blot showed that overexpression of miR-9 could down-regulate the expressions of TMEM16A mRNA and protein. The proliferation and invasion of T98G cells were suppressed after transfection of miR-9 mimics. Conclusion miR-9 may negatively regulate TMEM16A expression in glioma T98G cells and inhibit cell proliferation and invasion.