Abstract: Objective To investigate the relationship of serum cytokine signaling negative regulation factor-3 (SOCS-3), tumor necrosis factor-α (TNF-α), interleukin-16 (IL-16) and T helper cell 1/T helper 2 (Th1/Th2) ratio with hypertensive disorder complicating pregnancy (HDCP). Methods From January 2014 to June 2016, 182 cases with hypertensive disorder complicating pregnancy were selected from the department of obstetrics and gynecology in our hospital,including 51 cases of hypertensive of pregnancy, 69 cases of mild preeclampsia and 62 cases of severe preeclampsia. Another 65 cases of healthy pregnant women were selected as the controls.The levels of serum SOCS-3, TNF-α, IL-16 and Th1/Th2 in each group were detected by ELISA assay, and the relationship between the adverse pregnancy outcome and the serum indexes in patients with severe preeclampsia were analyzed. The correlation of serum SOCS-3 levels with TNF-α, IL-16 and Th1/Th2 were examined by spearman correlation analysis. Results Compared with the control group, the serum levels of SOCS-3 were significantly lower in the mild preeclampsia group and the severe preeclampsia group (0.59±0.12 ng/L vs 0.14±0.03 ng/L and 0.08±0.01 ng/L, P< 0.05). The levels of TNF-α, IL-16 and Th1/Th2 were 19.13±4.36, 143.26±57.82, 16.58±3.64 in gestational hypertension group, 34.75±6.42, 187.63±81.47, 20.15±4.82 in mild preeclampsia group, and 61.53±9.26, 284.62±113.58, 26.48±6.13 in severe preeclampsia group, which increased by disease severity (P < 0.05, respectively), and were significantly higher than those in control group (12.58±2.37, 108.47±35.24, 9.27±2.38)(P < 0.05, respectively). The incidences of fetal distress syndrome, fetal growth restriction and neonatal asphyxia in SOCS-3 low expression group were significantly higher than those in the high expression group (P < 0.05, respectively). The incidences of premature birth and fetal growth restriction in TNF-α, IL-16 and Th1/Th2 high expression groups were significantly higher than those in the low expression groups (P< 0.05, respectively). Correlation analysis showed that serumSOCS-3 level was negatively correlated with TNF-α, IL-16, Th1/Th2 ratio (r=-0.528, r=-0.506, r=-0.126; P< 0.05, respectively) in HDCP. Conclusion Monitoring maternal serum SOCS-3, TNF-α, IL-16 and Th1/Th2 will benifit the early detection, diagnosis, and treatment for hypertensive disorder complicating pregnancy, especially for severe preeclampsia patients.