Abstract: Objective To discuss the effect of exercise prescription on recovery of patients undergoing laparoscopic partial nephrectomy. Methods Totally 79 patients who underwent laparoscopic partial nephrectomy from February to June in 2016 in Chinese PLA General Hospital were included, and they were randomly divided into two groups, exercise prescription group and traditional education group. In traditional education group, routine health education was performed; while in exercise prescription group, exercise prescription was introduced, and the content of exercise prescription was constructed and performed by the rehabilitation team consisting of rehabilitation doctor, urologist and nurse. Post-operative recovery outcomes were compared between two groups. Results There were 42 cases in traditional education group with 29 males and 13 females, with an average age of (53.93±13.12) years, and 37 cases in exercise prescription group with 25 males and 12 females, with an average age of(50.54±12.73) years. There was no statistically significant difference in age, sex, BMI, surgical approach and side of operation between two groups. However, anal exhaust time and defecating time of patients in exercise prescription were shorter than those of patients in traditional education group (30.24±8.20) h vs (42.59±7.15) h, (2.70±1.05) d vs (3.74±0.77) d, P< 0.05, respectively. The rate of abdominal distension on the second day after operation, the mean post-operative hospital stay and ADL score on the third day in exercise prescription group were all significantly lower or shorter than those of traditional group 35.14% vs 59.52%, (4.35±1.95) d vs (5.33±1.56) d, (45.86±5.50) vs (54.48±6.75), all P< 0.05. Conclusion Application of exercise prescription in patients undergoing laparoscopic partial nephrectomy during the perioperative period can improve the recovery of gastrointestinal function after operation, shorten the post-operative hospital day and accelerate the regain of self-care ability.