Abstract: Objective To detect the correlation between the expression level of long non-coding RNA SNHG3 in glioma tissues and the clinicopathological factors of glioma, and examine the prognostic value of SNHG3 in patients with glioma. Methods Both glioma brain tissues of 70 patients and normal brain tissues of 30 cases with craniocerebral surgery who admitted to the department of neurosurgery, the Second Affiliated Hospital of Air Force Military Medical University from June 2008 to June 2013 were collected.The expression level of SNHG3 in glioma tissue samples and normal brain tissue samples was detected by RT-PCR, the survival rate was analyzed by Kaplan-meier method, and the factors affecting the postoperative prognosis in patients with glioma were also analyzed by univariate and multivariate analysis. Results Compared with normal brain tissue, the expression level of SNHG3 in glioma tissue increased significantly (1.000±0.297 vs 2.764±0.280, P=0.001), and it was positively correlated with the WHO classification of tumor. Furthermore, it was also closely related to histological grading, KPS score and postoperative recurrence, but it was unrelated with age, sex, tumor size and tumor number of glioma patients. Follow-up results showed that the 5-year survival rate of SNHG3 high expression group was significantly lower than SNHG3 low expression group (31.4% vs 63.0%; P=0.012). The high expression of SNHG3, high histological grade, KPS score＜ 80 and postoperative recurrence were the risk factors for prognosis of patients with glioma (all P＜ 0.05). Conclusion The expression level of SNHG3 is significantly up-regulated in glioma tissues, and the high expression level of SNHG3 is closely related to the poor prognosis of glioma patients.