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吴建国, 罗建飞, 刘家盛, 刘丹, 邓俊, 钱志成, 胡利萍, 李士军, 肖喆, 王旭峰, 彭志洋, 闫瑞承. 重型/ 危重型新型冠状病毒肺炎患者多种生物样本核酸检测结果分析[J]. 解放军医学院学报, 2020, 41(3): 205-207, 211. DOI: 10.3969/j.issn.2095-5227.2020.03.001
引用本文: 吴建国, 罗建飞, 刘家盛, 刘丹, 邓俊, 钱志成, 胡利萍, 李士军, 肖喆, 王旭峰, 彭志洋, 闫瑞承. 重型/ 危重型新型冠状病毒肺炎患者多种生物样本核酸检测结果分析[J]. 解放军医学院学报, 2020, 41(3): 205-207, 211. DOI: 10.3969/j.issn.2095-5227.2020.03.001
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WU Jianguo, LUO Jianfei, LIU Jiasheng, LIU Dan, DENG Jun, QIAN Zhicheng, HU Liping, LI Shijun, XIAO Zhe, WANG Xufeng, PENG Zhiyang, YAN Ruicheng. Detection of 2019-nCoV by real-time RT-PCR using multiple biological samples in severe/critically ill patients[J]. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2020, 41(3): 205-207, 211. DOI: 10.3969/j.issn.2095-5227.2020.03.001
Citation: WU Jianguo, LUO Jianfei, LIU Jiasheng, LIU Dan, DENG Jun, QIAN Zhicheng, HU Liping, LI Shijun, XIAO Zhe, WANG Xufeng, PENG Zhiyang, YAN Ruicheng. Detection of 2019-nCoV by real-time RT-PCR using multiple biological samples in severe/critically ill patients[J]. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2020, 41(3): 205-207, 211. DOI: 10.3969/j.issn.2095-5227.2020.03.001
shu

重型/ 危重型新型冠状病毒肺炎患者多种生物样本核酸检测结果分析

Detection of 2019-nCoV by real-time RT-PCR using multiple biological samples in severe/critically ill patients

    摘要
  • 摘要:
      目的  分析重型/危重型2019冠状病毒病(coronavirus disease 2019，COVID-19)患者多种生物样本核酸检测阳性率，并探讨其临床意义。
      方法  分析武汉大学人民医院东院感染性疾病五病区2020年1月31日- 2020年2月19日定点收治的重型/危重型COVID-19确诊病例，比较患者鼻咽拭子、痰液、血液、粪便、肛门拭子的病毒核酸实时荧光逆转录聚合酶链反应检测的阳性率。
      结果  共纳入19例重型/危重型COVID-19病例。治疗两周后复测鼻咽拭子新型冠状病毒(2019-nCoV)核酸检测阳性率42.10%(8/19)，痰液2019-nCoV核酸检测阳性率68.42%(13/19)，血液2019-nCoV核酸检测阳性率5.26%(1/19)，粪便2019-nCoV核酸检测阳性率21.05%(4/19)，肛门拭子2019-nCoV核酸检测阳性率10.52%(2/19)。
      结论  重型/危重症COVID-19患者痰液2019-nCoV核酸检测阳性率高于鼻咽拭子，消化道(粪便/肛拭子)亦有病毒核酸检出。重型/危重型COVID-19病例多种生物样本检测能提高2019-nCoV核酸检出率，降低出院病例2019-nCoV假阴性率。

     

    Abstract:
      Objective  To analyze the positive rates of 2019-nCoV by real-time RT-PCR using various biological samples in severe/critically ill patients, and explore their clinical significance.
      Methods  The confirmed severe/critically ill cases of COVID-19 in the fifth ward of Renmin Hospital of Wuhan University from January 31 to February 19 in 2020 were tested for 2019-nCoV by real-time fluorescence reverse transcriptase polymerase chain reaction. Positive rates of different specimens including nasopharyngeal swabs, sputum, blood, feces and anal swabs were compared.
      Results  A total of 19 severe/critically ill COVID-19 cases were included. After two weeks' treatment, positive rate of 2019-nCoV in the nasopharyngeal swab specimens was 42.10% (8/19), and it was 68.42% (13/19) in the sputum, 5.26% (1/19) in the blood, 21.05% (4/19) in the feces, 10.52% (2/19) in the anal swab.
      Conclusion  The positive rate of 2019-nCoV nucleic acid from sputum specimens of severe/critical ill COVID-19 patients is higher than that of nasopharyngeal swabs, blood and fecal/anal swabs. The detection using multiple biological samples can increase the detection rate of 2019-nCoV infection and reduce the false negative rate in discharged cases.

     

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