Abstract:
Objective To evaluate the feasibility and success rate of autologous endometrial transplantation method in establishing adenomyosis animal model in rhesus monkeys.
Methods Six healthy female rhesus monkeys were randomly divided into operation modeling group (n=5) and blank control group (n=1). For the experimental group, the endometrium was taken out through laparotomy and implanted into the anterior wall of the uterus. Ultrasound guided puncture biopsy was done for the pathological examination at 9 months after operation.
Results In the operation modeling group, four monkeys were in good condition without serious complications, and one monkey died at 6.5 months after operation (autopsy confirmed that the cause of death was upper gastrointestinal obstruction caused by swallowing monkey hairs). Endometrial glands were found in the myometrium of the dead monkey and the biopsy tissues of three monkeys in the operation group; endometrial glands were not found in the myometrium of one monkey in the operation group and the monkey in the control group; the modeling success rate was 80% (4/5).
Conclusion It is feasible to establish the uterine adenomyosis model of rhesus monkeys by autologous endometrial implantation, with high success rate. The individual differences of different monkeys are the important factors for the success of modeling.
下载: