鼠降钙素基因相关肽两种不同检测方法比较

Two different methods for detection of calcitonin gene-related peptide in rats:A comparative study

摘要: 目的对放射免疫分析法(RIA)和酶联免疫分析法(ELISA)检测鼠降钙素基因相关肽(CGRP)的效能进行评价。方法分别采用RIA法和ELISA法检测CGRP标准品、质控品和100例正常MCAO模型大鼠血浆标本中CGRP浓度,作精密度和比对分析。结果 RIA法和ELISA法检测正常MCAO模型大鼠血浆中CGRP结果具有显著相关性(P<0.01),相关系数r分别为0.986和0.962。结论 RIA法和ELISA法检测CGRP结果一致,符合科研检测的要求。

Abstract: Objective To evaluate the efficiency of radioimmunoassay（RIA） and enzyme linked immunoassay（ELISA） in detecting calcitonin gene-related peptide（CGRP） in rats. Methods CGRP levels in standard substance and quality control of CGRP,100 plasma samples from normal rats and middle cerebral artery occlusion（MCAO） model of rats were measured by RIA and ELISA,respectively,and their precision rates were compared. Results The relative coefficient of CGRP in normal rats and MCAO model of rats detected by RIA and ELISA was 0.986 and 0.962,respectively（P＜0.01）. Conclusion The CGRP detected by RIA is consistent with that detected by ELISA,thus both RIA and ELISA can be used in detection of CGRP.