Abstract: Objective To provide the evidence for the rational use of antibiotics by studying the distribution and drug resistance of pathogens in patients with infection after artificial joint replacement. Methods Pathogens from 80 patients with infection admitted to our hospital in 2010 were routinely identified after artificial joint replacement.Sensitivity of drugs was tested with the K-B method but that of vancomycin was detected by E-TEST. Results The infection rate of coagulase-negative staphylococci（CNS） was the highest（38.75%） followed by S.aureus（18.75%）.The resistance rate of CNS and S.aureus to penicillin,erythromycin,cefoxitin was 77.42%,74.19%,54.84% and 100%,73.33%,33.33%,respectively.The sensitivity of CNS and S.aureus to vancomycin and linezolid was 100%. Conclusion The first two pathogens in patients with infection after artificial joint replacement are CNS and S.aureus which are highly resistant to penicillin and macrolides but not resistant to vancomycin and linezolid.