自体CIK细胞联合IL-2治疗难治性脾非霍奇金淋巴瘤1例
Treatment of refractory non-Hodgkin’s lymphoma of the spleen with combined autologous cytokine induced killer cells and IL-2: A case report
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摘要: 目的 观察自体细胞因子诱导的杀伤细胞输注联合IL-2治疗老年淋巴瘤的有效性和安全性。 方法 1例老年脾脏恶性淋巴瘤患者在经过手术切除脾脏原发肿瘤、8个疗程的R-CHOP方案免疫化疗后出现淋巴瘤肝脏转移,此时采集患者外周血单核细胞,在体外经干扰素-γ(IFN-γ)、白介素-2(IL-2)和抗CD3单克隆抗体诱导成CIK细胞,每次回输细胞数为2-3×109个,回输后应用IL-2 100mU/d,皮下注射,连续10d,28d为1个周期。观察治疗前后患者肝功能、肿瘤相关生物学指标及影像学变化。 结果 患者共完成8个周期的CIK细胞输注,每次回输后未出现不良反应,肝功能指标、LDH水平均降至正常,PET/CT检查示治疗后肝脏淋巴瘤转移灶消失,达到完全缓解。 结论 自体CIK细胞输注联合IL-2疗法对于清除治疗后淋巴瘤的微小残留灶和(或)转移灶安全有效。Abstract: Objective To observe the efficiency and safety of combined autologous cytokine induced killer（CIK） cells and IL-2 in treatment of splenic lymphoma in old patients. Methods Hepatic metastasis of splenic primary malignant lymphoma occurred in an old patient after splenectomy and 8 cycles of R-CHOP chemotherapy.Peripheral blood mononuclear cells（PBMC） were collected.CIK cells were induced with in vitro interferon gamma（IFN-γ）,IL-2 and anti-CD3 monoclonal antibody（mAb）.Liver function,tumor-related biological indexes and image changes were observed after 2-3×109 CIK cells were re-transfused into the patient each time and IL-2 100mU/d was subcutaneously injected for 10 days,28 days a cycle. Results No adverse reaction occurred in the patient after 8 cycles of CIK cells transfusion.The liver function and serum LDH level became normal（P＜0.05）.PET-CT showed that hepatic metastasis of lymphoma disappeared and completely relieved. Conclusion Combined autologous CIK cells and IL-2 is safe and effective for small residual or metastatic foci of lymphoma in old patients after splenectomy.
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