Abstract: Osteoblasts are a kind of special cells with osteogenic potential, which play an important role in bone reconstruction and homeostasis. Osteoblast differentiation is regulated by multiple factors. Non-coding RNAs (ncRNAs), especially microRNAs and long non-coding RNAs (lncRNAs), are of great importance in the proliferation, differentiation, mineralization and apoptosis of osteoblasts. It has been proved that microRNAs and lncRNAs can regulate each other and form a complex biological regulatory network. However, the microRNAs-lncRNAs interaction in osteoblasts has not been fully clarified. Recent advances in the microRNAs, lncRNAs and their interactions in osteoblasts are reviewed in this paper.